FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_w5_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_w5_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15131491
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAATTCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA396990.2623601335783764No Hit
GCCAATTAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACT325300.21498211907868164No Hit
GCCAATTGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA284600.18808457144110913No Hit
GCCAATTCAACAGCGTCTTCGGTGTAACCCAAAACACCCTTCAACTTACC284350.187919353089527No Hit
GCCAATTCTTCACCAGCGTATCTACCGTGAGTGGAGTCGTACTTGAACAT236770.15647499641641396No Hit
GCCAATTGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG205270.1356574841170642No Hit
GCCAATTCGAAACGTGTGAATCAACAGAATCAACCACAGCATATCATAAA175560.11602293521504259No Hit
GCCAATTTGAAATTGTAGGTCACCAATAGCGTTAATTGGTATATTTCTTT171130.11309526602500705No Hit
GCCAATTCCACCGTTCAAAACGTTCAAGAATGGAACTGGCAAAACGTATG168960.11166117073327407No Hit
GCCAATTACTTCACCAGCGTATCTACCGTGAGTGGAGTCGTACTTGAACA164640.10880619761793467No Hit
GCCAATTTTCTTGAACAGTCAATTGCAAAGAACCGTCACCAATGAATAAG163770.10823123775442883No Hit
GCCAATTAGTTTCGGTGCTGGATGGAGAAACAGTTTCAACGGTGGAAGAT152770.10096163028481464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATTA3091500.069.2558752
GCCAATT11244400.069.2048651
CAATTAG901000.068.27343
GCCAATA2690000.068.038431
CCAATTG3800250.066.415432
CAATTAC531000.066.0838853
CCAATTC3577200.065.66012
CAATTAA904300.064.641243
CAATTAT929000.064.33553
CCAATAC671350.063.964732
AATTAGC412900.063.2068144
CCAATAG1263400.063.1836932
GCCAATC372850.062.6073761
CAATTGT1504800.061.7810133
GCCAATG886100.061.739981
AATTCGC166550.061.66214
CCAATTT1327950.061.32232
CAATTCG773250.061.278513
AATTACG109650.060.556614
CAATAGG338150.060.4000363
Produced by FastQC (version 0.11.2)