FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_w4_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_w4_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13105860
Sequences flagged as poor quality0
Sequence length76
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACCATCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA622270.47480287443937297No Hit
TGACCATCTCCGTTTCAAAGGCCTGATTTTATGCAGGCCACCATCGAAAG610700.465974762434514No Hit
TGACCATCTCGCATTTCACTGGGCCAGCATCAGTTTTGGTGGCAGGATAA561070.428106205926204No Hit
TGACCATAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACT447100.34114510608231735No Hit
TGACCATGTTATCTTTTCTTCTTAACAGCTTATCACCCCGGAATTGGTTT296390.22615074478134206No Hit
TGACCATATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC264210.20159684293896016No Hit
TGACCATCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCG257520.1964922561358049No Hit
TGACCATCTGCGACGTAAGTCAAGGATGCTGGCATAATGGTTATATGCCG248940.18994556633444887No Hit
TGACCATGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTG226280.1726555907052265No Hit
TGACCATCTTTTCTTCTTAACAGCTTATCACCCCGGAATTGGTTTATCCG214880.16395719166845976No Hit
TGACCATAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAG214830.1639190407954915No Hit
TGACCATAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTAT213170.16265243181294473No Hit
TGACCATGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG204330.15590735747215367No Hit
TGACCATCTCGCAAGAGGTGCACAATCGACCGATCCTGATGTCTTCGGAT183090.13970086663523035No Hit
TGACCATCTTCACCAGCGTATCTACCGTGAGTGGAGTCGTACTTGAACAT171630.13095668655090165No Hit
TGACCATGTCGACATGTAGTTAAACCACTATTCAGTCGGAACAATTGGTA168120.12827849526852875No Hit
TGACCATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAG166410.12697373541301374No Hit
TGACCATCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCTGCCGAAG164220.12530272717700325No Hit
TGACCATAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTA162510.12399796732148825No Hit
TGACCATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAA162330.12386062417880246No Hit
TGACCATCTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCT160140.122189615942792No Hit
TGACCATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGC155290.11848898126486931No Hit
TGACCATCTTCGGCGCCAGTGAAATACCACTACCTTTATAGTTTCTTTAC154950.11822955532868502No Hit
TGACCATATCTTTTCTTCTTAACAGCTTATCACCCCGGAATTGGTTTATC145760.11121742487711604No Hit
TGACCATGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATC133330.1017331178572028No Hit
TGACCATCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGAC132790.10132108842914543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCATG2839750.069.323772
TGACCAT9740900.069.277031
GACCATA2299200.068.863892
GACCATC3783600.068.213152
TGACCAG1827500.068.07831
ACCATGC618700.068.0070953
ACCATGT1263900.067.4867863
GACCAGG474700.067.349682
ACCATAT824600.066.795123
GACCATT1016050.066.790042
GACCAGC708200.065.496682
ACCATCT2030050.065.450543
CCATCTC677450.065.347414
GACCAGA441800.064.925752
CATCTCG191600.064.869265
CCATGCC254500.064.2675554
CAGCTCG43300.063.939975
ACCATAG651500.063.6054533
GACCAGT296200.063.5756722
CCATGCG121800.063.364374
Produced by FastQC (version 0.11.2)