FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_w3_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_w3_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11787933
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGATCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA317710.26952138258675207No Hit
ACTTGATAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACT309360.2624378676057965No Hit
ACTTGATCTCCGTTTCAAAGGCCTGATTTTATGCAGGCCACCATCGAAAG254020.2154915539475835No Hit
ACTTGATCTCGCATTTCACTGGGCCAGCATCAGTTTTGGTGGCAGGATAA223850.18989758424992745No Hit
ACTTGATATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC202870.17209972265705956No Hit
ACTTGATCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCG165380.1402960128802904No Hit
ACTTGATCTTCACCAGCGTATCTACCGTGAGTGGAGTCGTACTTGAACAT147110.1247971124369302No Hit
ACTTGATGTTATCTTTTCTTCTTAACAGCTTATCACCCCGGAATTGGTTT143150.12143774485314772No Hit
ACTTGATCAACAGCGTCTTCGGTGTAACCCAAAACACCCTTCAACTTACC141240.11981744382157583No Hit
ACTTGATCGAAACGTGTGAATCAACAGAATCAACCACAGCATATCATAAA128340.10887404941986012No Hit
ACTTGATGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATC125610.10655812176740402No Hit
ACTTGATGTCGACATGTAGTTAAACCACTATTCAGTCGGAACAATTGGTA122920.10427612712084469No Hit
ACTTGATAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTA120700.10239284529357268No Hit
ACTTGATCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAG120370.10211289799492414No Hit
ACTTGATCTCGCAAGAGGTGCACAATCGACCGATCCTGATGTCTTCGGAT120110.10189233345659497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGAT7643500.069.291091
TTGATCG663150.068.905843
CTTGATC3386400.068.892992
ACTTGAG2450950.068.721321
TGATCCC180450.067.655794
TGATCGG238600.067.626344
TTGATCT1593600.067.1088263
CGAGCGG1200.067.0840212
TTGATCC558800.066.9970863
TGATCGA137950.066.374534
CTTGAGG673600.066.331362
CTTGAGA740200.066.241042
CTTGATG2207250.066.1611942
GATCTCC149850.065.7987065
TTGATGC486700.065.4657443
TGATCTC525800.065.4096154
CTTGATA1963600.065.3829652
GATCCCG75950.065.210255
TGATCGC168350.064.9506154
GAGCTCG50900.064.776935
Produced by FastQC (version 0.11.2)