FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_w2_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_w2_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13850778
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATGTTCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA533100.384888126861899No Hit
CGATGTTCTCGCATTTCACTGGGCCAGCATCAGTTTTGGTGGCAGGATAA315020.22743848757087867No Hit
CGATGTTCTCCGTTTCAAAGGCCTGATTTTATGCAGGCCACCATCGAAAG282370.2038658045057108No Hit
CGATGTTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGC194870.14069245785327003No Hit
CGATGTTGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG192810.13920517677779543No Hit
CGATGTTAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACT186690.1347866524176476No Hit
CGATGTTGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA170850.1233504717207943No Hit
CGATGTTAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTAT144550.10436236866983213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTT9352350.069.7332841
CGATGTG2136350.069.5774151
GATGTTC3492550.068.926722
CGATGTA1538950.068.595121
GATGTGC845300.068.4459842
GATGTTA2146000.068.274892
GATGTTG3130800.068.08412
ATGTTCG540850.067.6695253
ATGTTCT1918850.066.901143
ATGTTAT873750.066.889153
ATGTTGC600600.066.176873
ATGTTAG564700.065.363183
ATGTTCC550250.064.846873
GTTCGGG37400.064.577135
GATGTTT831950.064.508042
ATGTTGT1377950.064.226873
CGATGTC734250.063.2601781
GATGTGA564400.062.887342
ATGTTGA484200.062.1623733
ATGTGCC141700.061.9776653
Produced by FastQC (version 0.11.2)