FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_w1_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_w1_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10533746
Sequences flagged as poor quality0
Sequence length76
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCCTGTCGACATGTAGTTAAACCACTATTCAGTCGGAACAATTGGTA128170.12167561283516805No Hit
GTGGCCTCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA127430.12097310871175364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGCCG3945350.069.836061
GTGGCCT4649100.069.5470661
TGGCCGC1388700.069.37642
TGGCCGG1215900.069.194732
GGCCGCG328100.068.92873
GGCCGGG323050.068.911863
GCCGAGC125200.068.775744
TGGCCTG1646100.068.526552
TGGCCTC1510300.068.483862
GGCCGAG286250.068.47773
GGCCGCT562900.068.47653
TGGCCGA965500.068.360612
GGCCGGT430400.068.330883
TGGCCCG448100.068.319272
GGCCTGT597700.067.944793
GGCCTGC433550.067.93133
GTGGCCC1140400.067.7322541
TGGCCTA1155350.067.682792
GGCCGAT312550.067.497593
GGCCTAT363800.067.475753
Produced by FastQC (version 0.11.2)