FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_w12_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_w12_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15944896
Sequences flagged as poor quality0
Sequence length76
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTTATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCT773320.48499532389549604No Hit
TAGCTTTAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAA572520.35906160817856697No Hit
TAGCTTTCAAAGGTTATGGGACTCATCAACCAAGTTGGATTCAGTGGCTC545530.3421345614295634No Hit
TAGCTTTATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCT487040.305451976607436No Hit
TAGCTTTGCCCATTTGGCATTGGCGGCCAAGATATCTTGTAGGTTTGAGT383030.2402210713697976No Hit
TAGCTTTCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTC379640.23809499917716614No Hit
TAGCTTTGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTG326860.20499349760575422No Hit
TAGCTTTGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG294880.1849369227619923No Hit
TAGCTTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA281850.17676502875904615No Hit
TAGCTTTGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA272090.17064394775607192No Hit
TAGCTTTGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATA264530.16590261861852218No Hit
TAGCTTTGCGACAGCGGCGACAGCGGCGATAGAAGCGACAGTAGAAAATT254330.15950558724246303No Hit
TAGCTTTCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGT249590.15653284913241203No Hit
TAGCTTTCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTC216830.13598708953636324No Hit
TAGCTTTGTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGG213050.13361642496758838No Hit
TAGCTTTAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTT201800.12656087565575844No Hit
TAGCTTTGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAG198520.12450379105639824No Hit
TAGCTTTCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGAC196120.12299860720320784No Hit
TAGCTTTCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAG187730.11773673531642978No Hit
TAGCTTTAGCGGATTCAGCGGCAACAAAGTTAGCCAAAGCGACGAAAGCA185840.11655140303204235No Hit
TAGCTTTCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAG179870.11280725819723127No Hit
TAGCTTTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATG173110.108567657010745No Hit
TAGCTTTTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGT168160.10546321531353983No Hit
TAGCTTTGGAAGATTGAGTAGATGTAGCAGCAGAGCTGGAGGCTGGAGAT165780.10397057465912603No Hit
TAGCTTTAACAGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCT162120.10167516928301068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTTT12854550.069.5746151
AGCTTTC3576300.069.299642
AGCTTTG4815800.069.0689242
TAGCTTA2138400.068.87331
GCTTTGC1179300.068.635133
AGCTTAG1070150.068.136332
AGCTTAC419350.068.054062
AGCTTTA3265950.067.988062
GCTTTGT1836150.067.9609153
GCTTTCG694800.067.950953
GCTTTAT1198900.067.323293
GCTTTCT1577600.066.935643
GCTTTAG901950.065.582263
GCTTAGG257150.065.255833
GCTTAGT427150.065.056883
GCTTTCC599400.064.9375463
AGCTTTT1556100.064.826812
GCTTTGA792150.064.783443
GCTTTCA872400.064.445373
CTTTGCG317950.064.060534
Produced by FastQC (version 0.11.2)