FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_w11_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_w11_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16140635
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGTAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAA538750.3337848851671573No Hit
TTCGAGTATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCT444800.2755777576284948No Hit
TTCGAGTCAAAGGTTATGGGACTCATCAACCAAGTTGGATTCAGTGGCTC400520.24814389272788834No Hit
TTCGAGTGCCCATTTGGCATTGGCGGCCAAGATATCTTGTAGGTTTGAGT392280.24303876520347556No Hit
TTCGAGTATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCT311780.19316464315065673No Hit
TTCGAGTAAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGA277490.17192012581908953No Hit
TTCGAGTGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTG245630.15218112546377513No Hit
TTCGAGTGCGACAGCGGCGACAGCGGCGATAGAAGCGACAGTAGAAAATT239560.14842043079469922No Hit
TTCGAGTCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGT234030.1449942954536795No Hit
TTCGAGTCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGAC221660.13733040862394819No Hit
TTCGAGTAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTT195980.12142025391194337No Hit
TTCGAGTAGCGACAGCGGCGACAGCGGCGATAGAAGCGACAGTAGAAAAT194790.12068298428159735No Hit
TTCGAGTGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA188640.11687272526762424No Hit
TTCGAGTCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTC175480.10871939053203297No Hit
TTCGAGTGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG173150.10727582898690169No Hit
TTCGAGTTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGT168500.10439490143975129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGTG3327000.069.719852
TCGAGTC2679550.069.682312
TTCGAGG3772950.069.636621
TTCGAGT9534100.069.602681
TCGAGTA2525800.069.598932
CGAGTCT996200.069.578963
TCGAGGG1100500.069.4451752
CGAGTCA680100.069.067413
CGAGTCC459750.068.923013
CGAGTGG791200.068.906973
CGAGTGC890500.068.8799443
TCGAGAG711900.068.8495252
CGAGTAT693550.068.81813
CGAGTCG567450.068.788933
TTCGAGC1080600.068.7868651
CGAGTAG757150.068.765173
CGAGTGA577350.068.640023
CGAGGGG274850.068.552313
CGAGTGT1119450.068.5251543
TTCGAGA1464800.068.3234561
Produced by FastQC (version 0.11.2)