FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_w10_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_w10_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15815956
Sequences flagged as poor quality0
Sequence length76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATCTCGCATTTCACTGGGCCAGCATCAGTTTTGGTGGCAGGATAA1462850.9249203778766204No Hit
CTCAGATCTCCGTTTCAAAGGCCTGATTTTATGCAGGCCACCATCGAAAG1294810.8186732436534345No Hit
CTCAGATGTTATCTTTTCTTCTTAACAGCTTATCACCCCGGAATTGGTTT818510.5175216724173992No Hit
CTCAGATCTTTTCTTCTTAACAGCTTATCACCCCGGAATTGGTTTATCCG613920.3881649645459307No Hit
CTCAGATGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTG377770.23885372468158106No Hit
CTCAGATCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA363640.2299197089319166No Hit
CTCAGATCTTCGGCGCCAGTGAAATACCACTACCTTTATAGTTTCTTTAC356760.2255696715392987No Hit
CTCAGATCTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCT354980.22444422581853413No Hit
CTCAGATGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG340530.2153078827482828No Hit
CTCAGATCTGCGACGTAAGTCAAGGATGCTGGCATAATGGTTATATGCCG328090.20744240816046783No Hit
CTCAGATCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCTGCCGAAG295250.18667856688523918No Hit
CTCAGATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAG288270.18226530220493783No Hit
CTCAGATAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAA276470.17480448225829662No Hit
CTCAGATATCTTTTCTTCTTAACAGCTTATCACCCCGGAATTGGTTTATC269950.17068206310133893No Hit
CTCAGATGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA267190.16893698996127707No Hit
CTCAGATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCA266310.16838058982966314No Hit
CTCAGATCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTC255470.16152675184478257No Hit
CTCAGATATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCT254380.1608375744090335No Hit
CTCAGATCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAG244310.15447058653931509No Hit
CTCAGATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAA238300.15067062654954275No Hit
CTCAGATGGTTCCACGTCAACAGCAGTTGGACGTGGGTTAGTCGATCCTA231120.14613090729387462No Hit
CTCAGATATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCT229770.1452773389101487No Hit
CTCAGATGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATA223500.14131298797239952No Hit
CTCAGATCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTC220460.13939087842682416No Hit
CTCAGATGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGC219260.13863215097462336No Hit
CTCAGATCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGAC211290.13359293614625634No Hit
CTCAGATCTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTC202650.12813009849041057No Hit
CTCAGATCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAA201910.1276622165615534No Hit
CTCAGATGCCCATTTGGCATTGGCGGCCAAGATATCTTGTAGGTTTGAGT195880.12384961111424438No Hit
CTCAGATGTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGG188180.11898110996262255No Hit
CTCAGATAGCTCCGTTTCAAAGGCCTGATTTTATGCAGGCCACCATCGAA187490.1185448416776071No Hit
CTCAGATGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTA187170.1183425143570202No Hit
CTCAGATAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCG186910.11817812340904336No Hit
CTCAGATCTCGCAAGAGGTGCACAATCGACCGATCCTGATGTCTTCGGAT185780.11746365505822096No Hit
CTCAGATCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAG184380.11657847303065336No Hit
CTCAGATCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGAC183870.11625601386346801No Hit
CTCAGATGCTCCGTTTCAAAGGCCTGATTTTATGCAGGCCACCATCGAAA180700.11425170884390422No Hit
CTCAGATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGC180610.11419480428498917No Hit
CTCAGATGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA174620.1104074897527535No Hit
CTCAGATAACAGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCT172450.1090354576100237No Hit
CTCAGATGACTAATCGAACCATCTAGTAGCTGGTTCCTGCCGAAGTTTCC170110.10755593907823215No Hit
CTCAGATCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATT165550.10467277475986908No Hit
CTCAGATGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGA160890.10172638315382262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT12162900.069.787361
TCAGATC4345800.069.469722
CTCAGAC333750.069.211931
TCAGATG3948350.069.11432
CAGATCG782950.068.3100363
CTCAGAG2261750.068.053811
GATCTCC235050.067.7257845
CAGATCA796350.067.62653
CAGATCC635900.067.5053943
CAGATAG815050.067.066953
AGATCCC217500.066.8499454
CAGATGT1669250.066.2299963
CTCAGAA928050.066.179341
CAGATAA821350.066.1562043
TCAGATA2918850.065.931242
CAGATCT2315000.065.838893
TCAGATT1263450.065.720282
TCAGAGC754850.065.693652
CAGATGA618500.065.4883353
CAGAGAG159250.065.234853
Produced by FastQC (version 0.11.2)