FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_q9_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_q9_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18104364
Sequences flagged as poor quality0
Sequence length76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACGCTAACAGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCT1379830.7621532576344576No Hit
TTACGCTGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTG696550.3847414910570733No Hit
TTACGCTGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA556590.30743416338734686No Hit
TTACGCTCAGAAAGCTAAAAAAAAGAAAATAGAAAACAACCAGAAGAAAA362850.2004212906899132No Hit
TTACGCTCAAAAAATGTGTATTGTAACAAATTAAAAGGTAATGAGCCTCA354310.1957041959607087No Hit
TTACGCTACCGGAAGTGGACAAGACAATGATAGCCTTGGCCTTTTGTTCG350390.19353897215058205No Hit
TTACGCTCCAAAAAATGTGTATTGTAACAAATTAAAAGGTAATGAGCCTC317450.17534446390936462No Hit
TTACGCTCACTAGAGGAATATTGCGCCAACAATTGTGGGAGCGTCAAGAG295490.163214791748553No Hit
TTACGCTATAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTC287120.15859159703152234No Hit
TTACGCTGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAG283860.15679092620983537No Hit
TTACGCTGAGAATAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAG281450.15545975544901772No Hit
TTACGCTAAAAGAAATCATCAAATCATTCATTCTTCAGACTTATTTCTTA277210.15311777867479906No Hit
TTACGCTGTCTTGGACTTAGACAAGTCAGCCAAATGTTGGTACAATGGGA272530.15053276657495399No Hit
TTACGCTACAGAAAGCTAAAAAAAAGAAAATAGAAAACAACCAGAAGAAA260670.14398185984329523No Hit
TTACGCTGTTTGGAGAGATTGGGTGCAAAGCCTTGTAGACGTTGTAAACT247610.13676812949629163No Hit
TTACGCTGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG238380.1316699111882638No Hit
TTACGCTGGTACCGGAAGTGGACAAGACAATGATAGCCTTGGCCTTTTGT234640.12960411092043886No Hit
TTACGCTCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA233610.12903518731726782No Hit
TTACGCTGTTTGCTTCAAAACTTTGTAAATATAAGAGGAATAGGTCTCCT230420.12727318120647596No Hit
TTACGCTACAGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCTT225710.12467159851624725No Hit
TTACGCTATTGGGTGCAAAGCCTTGTAGACGTTGTAAACTTGTTCTGGCT224110.12378783369578739No Hit
TTACGCTCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTC220180.12161708635553285No Hit
TTACGCTCTTGGTTTCTTCCAAGTATTGGACTTCGATGACTGGGTTACCA201370.11122732618500158No Hit
TTACGCTAGAATAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGC197970.10934932594152437No Hit
TTACGCTGGTTGAACGGCAATACCGAAGGTGGAAGCGTAGTCGGTAACAC194190.10726143155318794No Hit
TTACGCTCTTGGTTTCTTCCAAGTATTGGACTTCAATGACTGGGTTACCA193510.1068858315044925No Hit
TTACGCTCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGAC189090.10444443118797213No Hit
TTACGCTGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCTTCGG188920.10435053117579829No Hit
TTACGCTGTCTTGGTTTCTTCCAAGTATTGGACTTCGATGACTGGGTTAC185720.10258300153487855No Hit
TTACGCTCTAAAGTAGTGGTGGTAGCTTGGATTTGACCATCACTGATTTG182150.1006111012792275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG5558300.069.920991
TTACGCT10630450.069.902911
TACGCTC2963450.069.769052
TACGCTA2839100.069.7550052
TACGCTG4009600.069.687522
TACGCGG1914650.069.661592
TACGCGA1414450.069.601922
TACGCGC1701550.069.585632
TACGCGT616200.069.3293152
TTACGCA734200.069.153271
ACGCTAA874000.069.0748143
TTACGCC826400.069.0340351
ACGCTGT1413900.068.857743
ACGCTAT721750.068.849723
ACGCGAG429250.068.813413
ACGCTCT1151450.068.809283
ACGCGCT642400.068.641783
ACGCGTG281200.068.584933
ACGCGGT708350.068.551183
ACGCGGG512650.068.454053
Produced by FastQC (version 0.11.2)