FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_q8_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_q8_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11717593
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATTATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCT477430.40744716086315674No Hit
TCTGATTGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA273150.23311101520593863No Hit
TCTGATTAACAGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCT223940.19111433551242135No Hit
TCTGATTCTTATGTATAATTCTTCTTTGATGTCTGCATACAAATATGCCG208080.1775791325061384No Hit
TCTGATTCCACCGTTCAAAACGTTCAAGAATGGAACTGGCAAAACGTATG204210.17427640642579068No Hit
TCTGATTTGAAATTGTAGGTCACCAATAGCGTTAATTGGTATATTTCTTT192540.16431702312923824No Hit
TCTGATTCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA180290.15386265762942952No Hit
TCTGATTGTATATGTTGAGCAAGTGTGTTGATGTAGTATAGTAAGTCAAA174890.14925420263359548No Hit
TCTGATTCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGT173090.14771805096831747No Hit
TCTGATTGTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGG167420.14287917322269172No Hit
TCTGATTAGTATATGTTGAGCAAGTGTGTTGATGTAGTATAGTAAGTCAA158870.13558245281262116No Hit
TCTGATTAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTT141390.12066471330758802No Hit
TCTGATTATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCT130780.11160995265836593No Hit
TCTGATTATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTC129360.11039809967797994No Hit
TCTGATTCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAG129250.11030422374287961No Hit
TCTGATTGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG127820.10908383658657542No Hit
TCTGATTCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAA126380.10785491525435302No Hit
TCTGATTCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAG121230.10345981465647425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATTA2544450.069.48552
TCTGATT8604000.069.341451
CTGATTC2690100.069.314572
CTGATTG2616100.068.984622
CTGATAG691700.067.212152
TCTGATA1689000.067.176151
TGATTCC418800.067.004163
TGATTAT844750.066.2381443
TCTGATG895700.066.18771
TGATTCG568250.065.982343
GATTCGC126800.065.669594
TGATTAA757300.065.405723
TGATTCT1170350.065.297153
CTGATGC270400.065.267692
CTGATAC414650.065.0418852
GATTAGC286850.065.037154
TGATTCA691350.064.896133
TGATTAG768500.064.894313
GATTAGG120350.064.79754
ATTCGCG37650.064.704065
Produced by FastQC (version 0.11.2)