FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_q7_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_q7_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17993337
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACAGTCAAAGGTTATGGGACTCATCAACCAAGTTGGATTCAGTGGCTC943570.5243996708337092No Hit
TCACAGTAACAGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCT839250.4664226541191331No Hit
TCACAGTACAAAGGTTATGGGACTCATCAACCAAGTTGGATTCAGTGGCT822300.45700250042557417No Hit
TCACAGTGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTG783910.43566682489190306No Hit
TCACAGTAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAA689650.38328076665267813No Hit
TCACAGTATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCT597090.33183950258920847No Hit
TCACAGTAAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGA476960.2650759000401093No Hit
TCACAGTCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA411710.22881247652950645No Hit
TCACAGTCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGT368290.2046813217581597No Hit
TCACAGTGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA306020.1700740668615277No Hit
TCACAGTAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTT295890.16444420509658658No Hit
TCACAGTCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTC294820.16384954052714068No Hit
TCACAGTATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCT264060.14675432355877066No Hit
TCACAGTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA251450.13974617381978674No Hit
TCACAGTCAGAAAGCTAAAAAAAAGAAAATAGAAAACAACCAGAAGAAAA249880.13887362861041286No Hit
TCACAGTCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAA231770.128808791832221No Hit
TCACAGTACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGA231490.12865317867386133No Hit
TCACAGTCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGAC230280.1279807075252356No Hit
TCACAGTGCCCATTTGGCATTGGCGGCCAAGATATCTTGTAGGTTTGAGT230200.1279362466228471No Hit
TCACAGTACCGGAAGTGGACAAGACAATGATAGCCTTGGCCTTTTGTTCG221560.12313446916489143No Hit
TCACAGTCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCG210630.11705999837606554No Hit
TCACAGAGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTG208890.11609297374911612No Hit
TCACAGTTAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTCC201100.11176359337903803No Hit
TCACAGTGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATA194070.10785659158165048No Hit
TCACAGTGTGAAGTATGGAGCAATGACTAAGGTCTTGGCATCACCAACAT193100.10731750314019017No Hit
TCACAGTCCACCGTTCAAAACGTTCAAGAATGGAACTGGCAAAACGTATG189890.10553350943185247No Hit
TCACAGTACAGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCTT189740.10545014523987406No Hit
TCACAGTGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG188230.10461094570729154No Hit
TCACAGTCAAAAAATGTGTATTGTAACAAATTAAAAGGTAATGAGCCTCA183850.10217671130152232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACAGT12622700.069.828781
CACAGTG4481900.069.534942
TCACAGG2016250.069.311631
TCACAGA2445100.069.2758261
CACAGTC3803250.069.071732
CAGTGCG288600.068.8525164
ACAGTGC1097500.068.764853
CACAGAG1208800.068.71442
CACAGTA3394300.068.698282
CACAGTT1234550.068.63362
CACAGGG627550.068.538932
ACAGTCC617700.068.4528353
CAGTCCC164200.068.3633044
ACAGTCG815000.068.3004463
ACAGTGT1406050.068.091113
ACAGGGG173750.067.406723
ACAGTCT1427900.067.3261643
CAGTGCA224150.067.084594
CAGTCCG166400.067.017734
CACAGGC657800.066.903612
Produced by FastQC (version 0.11.2)