FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_q6_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_q6_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16938723
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCGATCAAAGGTTATGGGACTCATCAACCAAGTTGGATTCAGTGGCTC728050.429813983025757No Hit
TATCGATATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCT482530.28486799152450865No Hit
TATCGATATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCT467510.27600073512035117No Hit
TATCGATAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAA466790.2755756735616965No Hit
TATCGATAACAGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCT425120.25097523585455644No Hit
TATCGATGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA376710.22239574966778786No Hit
TATCGATGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTG334460.19745290126062043No Hit
TATCGATCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAA301300.17787645503146843No Hit
TATCGATCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA296540.17506632583814022No Hit
TATCGATACAAAGGTTATGGGACTCATCAACCAAGTTGGATTCAGTGGCT277450.16379629090103193No Hit
TATCGATCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGT253770.14981648852749999No Hit
TATCGATCAGAAAGCTAAAAAAAAGAAAATAGAAAACAACCAGAAGAAAA245920.14518213681161207No Hit
TATCGATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA234660.1385346463248735No Hit
TATCGATGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG218370.1289176285603112No Hit
TATCGATCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTC191990.11334384534182418No Hit
TATCGATGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAG188620.11135432110200988No Hit
TATCGATGGATTCAGTGGCTCTTTTGAAGAGTCAAAGAGTGACGATTCCT182220.10757599613619043No Hit
TATCGATGTTATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGAT181940.10741069441893582No Hit
TATCGATATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTC178030.10510237401013052No Hit
TATCGATGTTTGGAGAGATTGGGTGCAAAGCCTTGTAGACGTTGTAAACT177110.10455923979629396No Hit
TATCGATCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGAC170140.10044440776320623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAG5000900.069.875351
CGAGGGG48450.069.78754
TCGAGGG381450.069.638423
ATCGAGC1486750.069.623772
TCGAGCG310000.069.407033
CGATCCG115350.069.3063054
CGAGCCC60550.069.021514
TCGATGC936800.068.858393
ATCGATC3265550.068.7705462
CGGGCGC2400.068.5411536
ATCGAGG1725150.068.512142
CGAGCAG121900.068.338824
TATCGAC641950.068.324851
ATCGATG3851000.068.218712
TCGAGCT610750.068.1772463
CGATCGG228650.068.151934
GCGTCCG1750.067.9970867
CGATGCG191650.067.976974
CGAGCGG118650.067.762214
CGCGTGC3100.067.738829
Produced by FastQC (version 0.11.2)