FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_q5_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_q5_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15887284
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAACTAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACT874110.5501947343548463No Hit
TGAAACTCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA610420.3842192284093367No Hit
TGAAACTGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA464190.2921770643742505No Hit
TGAAACTAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTAT454820.2862792658581542No Hit
TGAAACTGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATC411930.25928283273591635No Hit
TGAAACTAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTA387190.24371063046396102No Hit
TGAAACTGTCGACATGTAGTTAAACCACTATTCAGTCGGAACAATTGGTA377780.2377876545795996No Hit
TGAAACTGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG331820.20885885844301647No Hit
TGAAACTCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCG282650.17790957850316014No Hit
TGAAACTCTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCC251590.15835935204532128No Hit
TGAAACTCTTGTCAGTGACACCGTACTTCTTAGCGACAGTGGCAGTGTCA245800.15471492798895015No Hit
TGAAACTCTTCACCAGCGTATCTACCGTGAGTGGAGTCGTACTTGAACAT243920.15353159168049116No Hit
TGAAACTCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAG238200.1499312280185839No Hit
TGAAACTGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTG209830.13207417957657205No Hit
TGAAACTCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGAC209750.13202382484004188No Hit
TGAAACTTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGT206130.12974527301205166No Hit
TGAAACTCAACAGCGTCTTCGGTGTAACCCAAAACACCCTTCAACTTACC198470.12492380698928779No Hit
TGAAACTCCACCGTTCAAAACGTTCAAGAATGGAACTGGCAAAACGTATG185190.11656492072527941No Hit
TGAAACTACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGA180120.11337368929767984No Hit
TGAAACTGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCT176710.11122731865308129No Hit
TGAAACTCTTCAGTGGTCAATGGGCACCAGGTGGTGTATTGAGTGATAAC165750.10432871974844787No Hit
TGAAACTTTCAGTGGTCAATGGGCACCAGGTGGTGTATTGAGTGATAACG165130.10393847054033906No Hit
TGAAACTCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAA159750.10055211450868506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAACT12242800.069.770691
GAAACTC4365350.069.499342
GAAACTA3079950.069.361382
TGAAACG2058750.068.461441
GAAACGC702450.068.033622
AAACTCT1826150.067.430533
GAAACTG3733700.067.240352
ACTCGGC102400.067.236815
AAACTCG852000.066.999363
AACTGTC375500.066.929444
AAACTAA1005150.066.834223
AAACTAG911000.066.675413
AAACTGA749600.066.6585853
AAACTAT743650.066.627083
ACTCGCG38000.066.505315
AAACTCA1172500.066.125723
GAAACTT1465300.066.084462
AACTCGC183800.066.02544
AACTAAC240300.065.9998554
AACTCGG252850.065.880284
Produced by FastQC (version 0.11.2)