FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_q4_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_q4_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14261891
Sequences flagged as poor quality0
Sequence length76
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTATCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA397730.27887606208741883No Hit
AGTCTATCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCG349350.2449534917915163No Hit
AGTCTATGTCGACATGTAGTTAAACCACTATTCAGTCGGAACAATTGGTA229320.16079214179942897No Hit
AGTCTATGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA224400.15734238888798127No Hit
AGTCTATCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAG215640.15120014589930605No Hit
AGTCTATATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCT207860.14574504881575662No Hit
AGTCTATATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCT207100.14521216015463867No Hit
AGTCTATCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAA170280.11939510686205636No Hit
AGTCTATCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAG156810.10995035651303184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAT9212300.069.82351
GTCTATC3353600.069.637472
CTACGCG8950.069.61154
AGTCTAG3204900.069.602221
GTCTATG3093000.069.464012
GTCTATA2123700.069.166552
GTCTAGG1026400.068.885272
CTATGCC271300.068.402824
TCTATGC753550.068.149763
CTATCCG127050.067.9914864
TCTATGT1302850.067.676533
CTATGTC410750.067.4717564
TCTATCT1630750.067.388823
TCTATGG673950.067.3803563
GTCTAGT503550.067.313762
AGTCTAC451700.067.242661
TCTATCC569700.067.2383353
GTCTAGA889750.067.2343442
CTATGTG282400.067.114724
GTCTATT1039450.066.959732