FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_q3_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_q3_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16794775
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAGTTAACAGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCT1023160.6092132821070839No Hit
ACAAGTTGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTG676260.40266094663370006No Hit
ACAAGTTCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA576500.34326152032402935No Hit
ACAAGTTGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA413540.24623134278369316No Hit
ACAAGTTCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTC387360.23064316134035734No Hit
ACAAGTTGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG284340.16930265514125672No Hit
ACAAGTTCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAA281060.16734966678624752No Hit
ACAAGTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA273210.16267559404636264No Hit
ACAAGTTAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACT263980.15717983718150438No Hit
ACAAGTTGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATA250520.14916543984661895No Hit
ACAAGTTAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTAT236320.14071042928529856No Hit
ACAAGTTCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGAC218960.13037388116244486No Hit
ACAAGTTCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAG216460.1288853229650293No Hit
ACAAGTTCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCG205860.12257383620798731No Hit
ACAAGTTACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGA204900.12200222986017972No Hit
ACAAGTTCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAG204020.12147825737468945No Hit
ACAAGTTGTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGG193870.11543471109318225No Hit
ACAAGTTAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTT193750.1153632602997063No Hit
ACAAGTTACAGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCTT179850.10708687672207576No Hit
ACAAGTTGTTTGGAGAGATTGGGTGCAAAGCCTTGTAGACGTTGTAAACT178240.10612824524294015No Hit
ACAAGTTATAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTC176170.10489571905548005No Hit
ACAAGTTAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAA175310.1043836550355691No Hit
ACAAGTTACCGGAAGTGGACAAGACAATGATAGCCTTGGCCTTTTGTTCG175050.10422884498303789No Hit
ACAAGTTGAGAATAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAG174000.10360365054012334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAGTT11891450.069.6118551
ACAAGTA1729350.068.802931
ACAAGTG2072650.068.761561
CAAGTTC3549650.068.7513662
CAAGTTG4694550.068.311892
CAAGTTA3041800.068.26792
AAGTTCG758350.067.545293
AAGTTGC966500.067.332693
CAAGTGG728650.067.220442
CAAGTGC611700.066.218512
CAAGTAG955100.065.993622
AAGTTCT1550200.065.889183
AGTTGCG206450.065.664474
AAGTTGT1715650.065.276243
AAGTTAT779000.065.2515263
AAGTTCA811500.065.092543
AGTTGCC355550.065.0923544
AAGTTGG1373650.064.853063
AAGTTAA911100.064.738313
CAAGTGA676950.064.432622
Produced by FastQC (version 0.11.2)