FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_q2_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_q2_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35842786
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTATGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTG1978920.5521110998458658No Hit
GACTTATAACAGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCT1956720.545917384881856No Hit
GACTTATCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA1593660.44462503556503674No Hit
GACTTATGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA808330.22552097373234326No Hit
GACTTATAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACT763650.2130554248768497No Hit
GACTTATATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCT714170.1992506944075162No Hit
GACTTATCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAA687360.19177080710188096No Hit
GACTTATGTTTGGAGAGATTGGGTGCAAAGCCTTGTAGACGTTGTAAACT676990.18887761682364756No Hit
GACTTATCTTGTCAGTGACACCGTACTTCTTAGCGACAGTGGCAGTGTCA618300.1725033316327587No Hit
GACTTATGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG585340.16330761788439102No Hit
GACTTATTTCTTGAACAGTCAATTGCAAAGAACCGTCACCAATGAATAAG557460.1555292046773373No Hit
GACTTATCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAG555310.15492936291280482No Hit
GACTTATCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGAC525220.14653436817104565No Hit
GACTTATAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTAT513310.14321152379170524No Hit
GACTTATAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAA493190.13759812086035944No Hit
GACTTATCCACCGTTCAAAACGTTCAAGAATGGAACTGGCAAAACGTATG489910.1366830134242355No Hit
GACTTATCAACAGCGTCTTCGGTGTAACCCAAAACACCCTTCAACTTACC488330.13624219947634653No Hit
GACTTATGTTCGTTTCAATTTTTGCTGTATTGTTCCAGGCAGAGCGGTTA486750.13580138552845752No Hit
GACTTATGTCTTGGACTTAGACAAGTCAGCCAAATGTTGGTACAATGGGA486510.1357344264477655No Hit
GACTTATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA426520.11899744623646163No Hit
GACTTATCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTC419030.11690776492653222No Hit
GACTTATGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATA396330.11057455187774745No Hit
GACTTATCCGTGATACAATGGGATCAGATGCGTATAGTTTAAAGAATTTG393860.10988543133895896No Hit
GACTTATACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGA385720.10761440251882207No Hit
GACTTATGTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGG377520.10532663392851215No Hit
GACTTATCTTCACCAGCGTATCTACCGTGAGTGGAGTCGTACTTGAACAT373870.10430829790965468No Hit
GACTTATATTGGGTGCAAAGCCTTGTAGACGTTGTAAACTTGTTCTGGCT372760.10399861216145419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTATG10123650.069.729742
GACTTAT28218200.069.597241
ACTTATA6725450.069.221662
CTTATGC2164050.068.6852653
CTTATGT4133800.068.683343
ACTTATC9085400.068.634822
ACTTAGG1204650.068.57642
CTTATGG2327250.068.4333953
GACTTAA2714050.067.999511
TTATGCC771950.067.914034
CTTATCG1796500.067.815133
CTTATAT2044250.067.3682563
CTTATGA1718550.067.241243
CTTATCC1487400.067.223543
TTATGCG477300.066.916984
CTTATAG2002000.066.800323
CTTATCT4140050.066.430493
CTTATAC1084300.066.421893
ACTTAAG1393050.066.103782
TTATCCC413250.065.574534
Produced by FastQC (version 0.11.2)