FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_q1_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_q1_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15586035
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAAATGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTG749140.48064822130837No Hit
TCGAAATAACAGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCT536820.3444237100712272No Hit
TCGAAATCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA473980.3041055662970088No Hit
TCGAAATCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAA450810.2892396943802577No Hit
TCGAAATAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACT400200.2567683185620974No Hit
TCGAAATCAAAGGTTATGGGACTCATCAACCAAGTTGGATTCAGTGGCTC389480.24989036660061392No Hit
TCGAAATACAAAGGTTATGGGACTCATCAACCAAGTTGGATTCAGTGGCT369980.23737916667067668No Hit
TCGAAATAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTAT318880.20459340685427693No Hit
TCGAAATCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGT302080.19381452691463866No Hit
TCGAAATCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTC298280.1913764469282919No Hit
TCGAAATCCACCGTTCAAAACGTTCAAGAATGGAACTGGCAAAACGTATG291130.18678900695398157No Hit
TCGAAATATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCT288070.18482571096497602No Hit
TCGAAATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA245930.15778868711638336No Hit
TCGAAATATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCT244790.15705726312047932No Hit
TCGAAATTAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTCC210020.13474883124540654No Hit
TCGAAATCTTGTCAGTGACACCGTACTTCTTAGCGACAGTGGCAGTGTCA197300.126587679291109No Hit
TCGAAATAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAA195670.12554187129696553No Hit
TCGAAATACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGA187280.12015884732711046No Hit
TCGAAATGAGAATAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAG182690.11721390334360213No Hit
TCGAAATTGAAATTGTAGGTCACCAATAGCGTTAATTGGTATATTTCTTT179710.11530193535430916No Hit
TCGAAATGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATC178110.11427537536005788No Hit
TCGAAATTTCTTGAACAGTCAATTGCAAAGAACCGTCACCAATGAATAAG169140.10852022339228676No Hit
TCGAAATCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGAC168070.10783371139613121No Hit
TCGAAATACAGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCTT165990.10649918340360456No Hit
TCGAAATGTTTGGAGAGATTGGGTGCAAAGCCTTGTAGACGTTGTAAACT162670.1043690714155332No Hit
TCGAAATGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG161060.10333609542131786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAAAT12455150.069.6413041
CGAAATG4404750.069.6216352
CGAAATC4187050.069.237982
CGAAATA2891050.069.140582
GAAATGC1200800.067.912433
GAAATCC857650.067.736193
GAAATCG915600.067.4898763
CGAAATT1220950.067.305662
TCGAAAG1611550.067.253321
GAAATCT1651500.066.622723
CGAAAAG518450.066.016132
GCCGCGC1650.065.7564959
GAAATGT1520950.065.566023
GCTCGCG3150.065.5546961
GAAATAG887750.065.191133
AAATGCG260050.064.863024
GAAATAC614600.064.520883
GAAATGG1193250.064.074043
CCCGGGA1750.063.9979178
GAAATGA779700.063.9179343
Produced by FastQC (version 0.11.2)