FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_q12_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_q12_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16297655
Sequences flagged as poor quality0
Sequence length76
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTACTTAACAGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCT941710.5778193243138353No Hit
TGTACTTATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCT863880.5300639877332046No Hit
TGTACTTCAAAGGTTATGGGACTCATCAACCAAGTTGGATTCAGTGGCTC673370.4131698701438949No Hit
TGTACTTACAAAGGTTATGGGACTCATCAACCAAGTTGGATTCAGTGGCT667570.40961107594927No Hit
TGTACTTATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCT607430.3727100616622453No Hit
TGTACTTAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAA606010.3718387706697681No Hit
TGTACTTCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAA386120.23691751972906533No Hit
TGTACTTGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTG377180.23143206798769517No Hit
TGTACTTCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGT369950.22699584694853336No Hit
TGTACTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA361020.22151653105922292No Hit
TGTACTTGGCAGCGGATTCAGCGGCAACAAAGTTAGCCAAAGCGACGAAA329960.20245857456180047No Hit
TGTACTTAAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGA317310.19469672170628227No Hit
TGTACTTGTGACATCGGTGGAAGAAGCAGATGTACTGTTGGAAAATTGGG305680.18756072576085334No Hit
TGTACTTGCAGCGGATTCAGCGGCAACAAAGTTAGCCAAAGCGACGAAAG301190.18480572818604885No Hit
TGTACTTAGCGGATTCAGCGGCAACAAAGTTAGCCAAAGCGACGAAAGCA295760.18147396051763276No Hit
TGTACTTGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA290110.1780072041039033No Hit
TGTACTTAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTT277890.17050919288695213No Hit
TGTACTTGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATA261670.16055684084612173No Hit
TGTACTTGCGGCAGCGGATTCAGCGGCAACAAAGTTAGCCAAAGCGACGA255300.156648303084094No Hit
TGTACTTCAGAAAGCTAAAAAAAAGAAAATAGAAAACAACCAGAAGAAAA249250.15293611258797662No Hit
TGTACTTCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTC241170.1479783441237405No Hit
TGTACTTGCCCATTTGGCATTGGCGGCCAAGATATCTTGTAGGTTTGAGT235520.14451158771001105No Hit
TGTACTTACAGAAAGCTAAAAAAAAGAAAATAGAAAACAACCAGAAGAAA217830.133657265416405No Hit
TGTACTTGTTATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGAT207170.12711644712076678No Hit
TGTACTTGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAG205550.12612243908709564No Hit
TGTACTTGGTTGAACGGCAATACCGAAGGTGGAAGCGTAGTCGGTAACAC198410.12174144071647117No Hit
TGTACTTGAGGAAGTGACATCGGTGGAAGAAGCAGATGTACTGTTGGAAA196610.1206369873457255No Hit
TGTACTTCCAAAAAATGTGTATTGTAACAAATTAAAAGGTAATGAGCCTC191840.11771018591324948No Hit
TGTACTTACAGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCTT186520.11444591261749006No Hit
TGTACTTCAAAAAATGTGTATTGTAACAAATTAAAAGGTAATGAGCCTCA182500.11197930008949139No Hit
TGTACTTCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCG182020.11168477919062589No Hit
TGTACTTCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTC177280.10877638531432895No Hit
TGTACTTGGAAGATTGAGTAGATGTAGCAGCAGAGCTGGAGGCTGGAGAT175740.10783146409713545No Hit
TGTACTTCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTAC175710.10781305654095637No Hit
TGTACTTGTCTTGGTTTCTTCCAAGTATTGGACTTCGATGACTGGGTTAC166590.10221715946251164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACTT12038900.069.2830661
TGTACTA1834700.069.264281
GTACTTC3150350.069.161962
GTACTTA3128050.068.985852
TACTTAG941100.068.108453
GTACTTG4899650.067.980342
TACTTCG722150.067.824163
TACTAGG282350.067.736683
GTACTAC322700.067.533282
GTACTAG1045650.067.475672
TACTTGC1245200.067.368593
TACTTCT1222100.067.2099153
ACTTCGC191650.067.155334
TACTTAC539750.066.86013
TACTTCA802350.066.7683263
ACTTGCC366250.065.9427644
TACTTAT874900.065.9405443
ACTTAGG160250.065.898114
ACTTGCG378250.065.794014
ACTTCGG304350.065.772224
Produced by FastQC (version 0.11.2)