FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_q11_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_q11_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14286283
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATGCTAACAGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCT423920.2967321870916319No Hit
AAATGCTAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAA347010.24289733025728244No Hit
AAATGCTACAAAGGTTATGGGACTCATCAACCAAGTTGGATTCAGTGGCT334650.234245674679691No Hit
AAATGCTATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCT306400.2144714618911021No Hit
AAATGCTATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCT301540.21106959731933073No Hit
AAATGCTCAAAGGTTATGGGACTCATCAACCAAGTTGGATTCAGTGGCTC265810.18605959296760397No Hit
AAATGCTGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTG233260.1632755000023449No Hit
AAATGCTGCCCATTTGGCATTGGCGGCCAAGATATCTTGTAGGTTTGAGT224000.15679375804049242No Hit
AAATGCTGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA197940.13855248422560298No Hit
AAATGCTCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAA169780.11884126892908392No Hit
AAATGCTAAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGA166560.11658735865725187No Hit
AAATGCTCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGT165550.11588038680180142No Hit
AAATGCTAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTT148920.10423985021156307No Hit
AAATGCTCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTC147810.1034628811427017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATGCG6802200.069.685851
AAATGCT6185750.069.610991
AATGCGC1840100.069.478932
AATGCGG2552400.069.42082
AATGCGA1756550.069.334212
AATGCTC1657300.069.1903842
AATGCTG2441400.069.1561052
ATGCGCG424500.069.083013
ATGCGGT854800.068.950063
AATGCTA1567850.068.8914642
ATGCGGG652350.068.88513
ATGCTCG383250.068.508683
ATGCGCT707150.068.363173
ATGCGAG528950.068.305843
ATGCGCA453600.068.1004943
ATGCGAT488400.067.720293
ATGCGGA451400.067.7189943
AATGCGT740650.067.668352
ATGCGTG319350.067.573243
AATGCCG444100.067.446122
Produced by FastQC (version 0.11.2)