FastQCFastQC Report
Tue 10 Jan 2017
HGGNWBGX2_n01_q10_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGNWBGX2_n01_q10_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19645286
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGTTAACAGTTTCAACGGTGGAAGATGGGGCAGCAGTGGTGGTAGCT853740.43457753681977446No Hit
GAACGTTGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTG829810.4223964975618069No Hit
GAACGTTAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAA803620.40906505509769625No Hit
GAACGTTCTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAA612450.3117541785851323No Hit
GAACGTTATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCT603820.3073612672271608No Hit
GAACGTTATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCT550400.2801689932129265No Hit
GAACGTTCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTC548300.2791000344815545No Hit
GAACGTTAAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGA412200.20982132812930288No Hit
GAACGTTGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATA377930.19237693968924657No Hit
GAACGTTCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAA377740.19228022437545578No Hit
GAACGTTGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATA335040.1705447301708919No Hit
GAACGTTAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTT333850.1699389868897811No Hit
GAACGTTATAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTC314620.16015037907821755No Hit
GAACGTTGTGAAGTATGGAGCAATGACTAAGGTCTTGGCATCACCAACAT309740.15766632259769597No Hit
GAACGTTGTTATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGAT309650.15762051008063716No Hit
GAACGTTCAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGAC304660.15508046052371038No Hit
GAACGTTAGCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTC272230.13857268354352284No Hit
GAACGTTACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGA267660.13624642573287046No Hit
GAACGTTCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGT263500.13412886938881927No Hit
GAACGTTGCCCATTTGGCATTGGCGGCCAAGATATCTTGTAGGTTTGAGT255710.13016354152339651No Hit
GAACGTTGAGAATAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAG240770.12255866369163575No Hit
GAACGTTGTCTTGGTTTCTTCCAAGTATTGGACTTCGATGACTGGGTTAC237560.12092468391653856No Hit
GAACGTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA236170.12021713504196374No Hit
GAACGTTCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCG235810.12003388497372855No Hit
GAACGTTGTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGG226820.11545772354752179No Hit
GAACGTTCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTC225770.11492324418183578No Hit
GAACGTTCAGAAAGCTAAAAAAAAGAAAATAGAAAACAACCAGAAGAAAA223570.11380338265373179No Hit
GAACGTTATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTC220140.1120574167258242No Hit
GAACGTTAGAATAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGC218170.11105463163020379No Hit
GAACGTTGGTTGAACGGCAATACCGAAGGTGGAAGCGTAGTCGGTAACAC213830.10884545025203501No Hit
GAACGTTGGAAGATTGAGTAGATGTAGCAGCAGAGCTGGAGGCTGGAGAT210030.10691114397621902No Hit
GAACGTTACCGGAAGTGGACAAGACAATGATAGCCTTGGCCTTTTGTTCG207410.10557749070184064No Hit
GAACGTTCTTGGTTTCTTCCAAGTATTGGACTTCAATGACTGGGTTACCA207010.10537387951491263No Hit
GAACGTTGTCTTGGTTTCTTCCAAGTATTGGACTTCAATGACTGGGTTAC206600.10516517804831144No Hit
GAACGTTGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAG205910.10481394875086064No Hit
GAACGTTTAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTCC203940.10381116365524024No Hit
GAACGTTGTTTGGAGAGATTGGGTGCAAAGCCTTGTAGACGTTGTAAACT199100.10134746829341146No Hit
GAACGTTCTTGGTTTCTTCCAAGTATTGGACTTCGATGACTGGGTTACCA197940.10075699585132027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGTA3089500.069.307371
GAACGTG1975500.069.107461
AACGTTG5309050.069.031222
ACGTTGC1163450.068.811863
ACGTTCG802500.068.479643
CGTTCCG159050.068.289664
AACGTAG1683250.068.171442
AACGTTA3456100.068.1420752
ACGTTAT951350.068.081973
GAACGTT13562800.067.821371
ACGTAGC382250.067.469733
CGTTCGG293250.067.034124
GAACGTC1157700.066.959761
AACGTGC561550.066.9595262
ACGTTCC628700.066.8940053
AACGTGG692150.066.8873442
ACGTTAG1148000.066.362633
AACGTCG532550.066.1296842
CGTTGCC437500.066.02194
ACGTTGG1465150.065.8278053
Produced by FastQC (version 0.11.2)