FastQCFastQC Report
Thu 31 Aug 2017
HGGMKBGX3_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGMKBGX3_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157253445
Sequences flagged as poor quality0
Sequence length75
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGACT866100.068.794374
AATTAGA41482100.068.7924548
TTGGGTC1203650.068.745294
GGGTCGC7305550.068.72086
GAATTAG41556000.068.7061347
ATTGGGG922950.068.6877752
GTGTCGC11086800.068.686976
TGGGGAC522050.068.68063
AGAGGGG990550.068.653232
GTACTCG12003500.068.643156
CCTCGAG1556550.068.6391758
GGGTCTC11128450.068.595146
TGAATTA41601550.068.5896546
CCTGTGT2672450.068.580853
GGGGTCT3095500.068.5628055
AAGGGGT499350.068.5625463
GGGCCTC2370950.068.5623865
GTGGACT1788950.068.553984
CAAGGGG497800.068.5475542
ACTCGCT13214500.068.506018
Produced by FastQC (version 0.11.2)