FastQCFastQC Report
Fri 18 Jan 2019
HGGLTBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGLTBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23349481
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1804780.772942233705323No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC528670.2264161674514307TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC459260.1966895966552747TruSeq Adapter, Index 11 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC417490.17880054807213916TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC379830.1626717099193768TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCTTTCCCTACACGA328770.14080398617853646TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC324770.1390908860029908TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC298740.12794288661062747TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC264960.11347575562814438TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC257610.11032793405557922TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG997600.064.9219370
CTCCAGT1557500.061.60095224
CAGTCAC1472950.061.39145727
CGTCTGA1630100.061.38855716
CTGAACT1637800.061.38390419
ACGTCTG1631650.061.37956615
GCACACG1637850.061.31655511
CACACGT1647300.061.21809812
GAACTCC1638350.061.1439521
ACACGTC1646600.061.14343313
CCAGTCA1512800.061.0654626
AGCACAC1670700.060.90797810
TGAACTC1651300.060.87531720
AACTCCA1665100.060.86661522
AGAGCAC1670550.060.645218
CACGTCT1659400.060.4186214
AGTCACC369800.060.14626728
TCGGAAG1686550.059.8627743
CGGAAGA1704100.059.5047344
ATCGGAA1712300.059.111992
Produced by FastQC (version 0.11.5)