FastQCFastQC Report
Fri 18 Jan 2019
HGGLTBGX9_n01_9_BZIP3_BIO_180_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGLTBGX9_n01_9_BZIP3_BIO_180_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16507218
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC282816717.13291119072881TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG4626672.8028163195033833TruSeq Adapter, Index 9 (100% over 49bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT279440.16928352191144505Illumina Single End PCR Primer 1 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC177020.1072379367619668TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG2701350.066.5997370
TATGCCG3687600.057.9635146
GTATGCC3688350.057.9418145
GCCGTCT3688200.057.90371749
CGTATGC3693000.057.8971544
ATGCCGT3694750.057.85579747
TGCCGTC3695750.057.81706248
TCTCGTA3670650.057.783941
CTCGTAT3689100.057.6592342
CCGTCTT3708550.057.65752850
TCGTATG3714450.057.6260443
TGCTTGA3718100.057.44233358
ATCTCGT3687700.057.35376440
GCTTGAA3733650.057.23871659
AGATCTC3673550.057.13678738
CTTGAAA3755850.057.0052760
TTCTGCT3753350.056.92927655
TCTGCTT3755950.056.90132556
CAGATCT3686100.056.78235637
CTGCTTG3762550.056.72466757
Produced by FastQC (version 0.11.5)