FastQCFastQC Report
Fri 18 Jan 2019
HGGLTBGX9_n01_8_RAV1_BIO_10_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGLTBGX9_n01_8_RAV1_BIO_10_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9929298
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC304782830.69530192366067TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG2789452.8093124005342576TruSeq Adapter, Index 8 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC1653201.6649716827916736TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC761500.7669222940030604TruSeq Adapter, Index 8 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTG615060.6194395615883419TruSeq Adapter, Index 8 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC593680.5979073243647235TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGTATGC454080.457313296468693TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGG414670.4176226758427434TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGTATG405190.4080751730887722TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGTTTG385700.388446393692686TruSeq Adapter, Index 8 (97% over 46bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT377580.38026857487810317Illumina Single End PCR Primer 1 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATG351420.3539223014557525TruSeq Adapter, Index 8 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGC338040.34044702858147674TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATGC322950.32524957957752904TruSeq Adapter, Index 8 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGTTTG281640.2836454299186106TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTTTG201280.2027132230294629TruSeq Adapter, Index 8 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTG184700.18601516441545013TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGC157110.1582287086156544TruSeq Adapter, Index 8 (98% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG155390.1564964612805457Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGTATGC154800.1559022601597817TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATG154110.15520734698465088TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGTATG153030.15411965679748962TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAAGC133160.13410817159480962TruSeq Adapter, Index 8 (98% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGT128240.12915313851996385Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGG109320.11009841783376831TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATT108420.10919200934446725TruSeq Adapter, Index 8 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCCTATGC105070.10581815552317998TruSeq Adapter, Index 8 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG3302850.068.7533270
TTCTGCT3775850.063.11873655
TCTGCTT3857200.062.93469656
CTGCTTG3798500.062.81369457
CTTCTGC3880500.062.76523254
TCGTATG3660900.062.7216743
TGCTTGA3795900.062.62808258
TCTTCTG3740950.062.58147453
CGTATGC3808450.062.5807344
TCTCGTA3754350.062.53777341
CTCGTAT3737900.062.46776242
ATGCCGT3735050.061.91506247
TATGCCG3862050.061.86957646
GTATGCC3932750.061.78827745
GTCTTCT3747350.061.69378752
ATCTCGT3896500.061.23164440
GCCGTCT3732650.061.05377249
CCGTCTT3827200.060.790450
GCTTGAA3948250.060.76277559
TGCCGTC3804900.060.694148
Produced by FastQC (version 0.11.5)