FastQCFastQC Report
Fri 18 Jan 2019
HGGLTBGX9_n01_7_CRF4_BIO_10_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGLTBGX9_n01_7_CRF4_BIO_10_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13291784
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC370037927.839596249833733TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG3039052.28641241837815TruSeq Adapter, Index 7 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC852560.6414187892310017TruSeq Adapter, Index 7 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTTTG415740.31277968405144113TruSeq Adapter, Index 7 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGGATGC307660.23146629526931825TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTTTGC240690.18108178706485148TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGG227050.17081980868783303TruSeq Adapter, Index 7 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATTC203880.1533879876471059TruSeq Adapter, Index 7 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATG194770.1465341296548304TruSeq Adapter, Index 7 (97% over 49bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT187420.1410043979047508Illumina Single End PCR Primer 1 (96% over 30bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC166700.12541582078071686TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTATGC153600.1155601084098267TruSeq Adapter, Index 7 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTATG141930.10678024861072073TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTTTG136870.10297338566440742TruSeq Adapter, Index 7 (97% over 46bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG3411600.068.37807570
TGCTTGA4341250.063.19663258
TTCTGCT4322150.063.08072355
TCTCGTA4389200.063.02467341
TCGTATG4368950.062.97160743
CGTATGC4445200.062.93716444
CTCGTAT4388450.062.9040742
CTGCTTG4352750.062.83028857
TCTGCTT4392050.062.7998556
TATGCCG4460450.062.70816446
GTATGCC4491700.062.69158645
ATGCCGT4400750.062.6598847
CTTCTGC4408100.062.59016454
TCTTCTG4373450.062.36757753
GCCGTCT4385400.062.2259349
CCGTCTT4420900.062.10681550
GCTTGAA4460000.062.0891259
TGCCGTC4440600.061.9617748
GTCTTCT4384900.061.9325552
ATCTCGT4508850.061.91447440
Produced by FastQC (version 0.11.5)