FastQCFastQC Report
Fri 18 Jan 2019
HGGLTBGX9_n01_6_BZIP3_BIO_10_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGLTBGX9_n01_6_BZIP3_BIO_10_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17174150
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC6328073.6846481485255453TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1333820.7766439678237351TruSeq Adapter, Index 6 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG725700.057.81855870
TATGCCG863600.055.212646
GTATGCC869800.054.88358345
CGTATGC868300.054.87551544
ATGCCGT869850.054.7389647
GCCGTCT868850.054.6703549
TGCCGTC870700.054.6167148
TCTCGTA882050.053.9416541
CCGTCTT885200.053.73901450
CTCGTAT888000.053.6185442
TCGTATG891400.053.53307343
TCACGCC875900.053.45596330
CGCCAAT872650.053.1208533
ATCTCGT896400.053.0993540
AGTCACG897900.053.08542328
ACGTCTG910250.053.0127815
CGTCTGA912400.052.85304316
CACGCCA885250.052.7917331
ACACGTC917950.052.75505413
GCCAATA880400.052.64328834