FastQCFastQC Report
Fri 18 Jan 2019
HGGLTBGX9_n01_5_RAV1_BIO_5_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGLTBGX9_n01_5_RAV1_BIO_5_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13954515
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC4353853.120029610488075TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC581060.41639569701992507TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC553150.066.8843444
TATGCCG552450.066.8604146
ATGCCGT550700.066.74094447
GCCGTCT547100.066.676849
GTATGCC557000.066.6417345
TGCCGTC549500.066.6057248
ATCTCGT492800.066.1242140
CCGTCTT556200.065.9629750
TGCTTGA563550.064.87833458
GTCACAC584250.064.7200429
GCTTGAA572100.064.35548459
TCGTATG572450.063.76858543
ACGTCTG607250.063.70789715
CGTCTGA606850.063.69222316
CTGAACT607950.063.60895519
ACACGTC612750.063.24067713
GCACACG612750.063.18379211
CACACGT613450.063.15094412
TCTCGTA518050.063.0328141
CTTGAAA588650.062.90875260
Produced by FastQC (version 0.11.5)