FastQCFastQC Report
Fri 18 Jan 2019
HGGLTBGX9_n01_4_CRF4_BIO_5_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGLTBGX9_n01_4_CRF4_BIO_5_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13701687
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC308977422.550318073971475TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG5639264.115741368197946TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC330490.2412038751140644TruSeq Adapter, Index 4 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC197060.1438217060424749TruSeq Adapter, Index 4 (100% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT197000.14377791581430813Illumina Single End PCR Primer 1 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCCAATCTCGTATGC150830.11008133523995986TruSeq Adapter, Index 4 (98% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG148880.10865815282453904Illumina Single End PCR Primer 1 (96% over 32bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT143000.10436671046419321TruSeq Adapter, Index 4 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG2973700.067.78725470
TGCTTGA4088150.057.18841658
TATGCCG4104500.057.15946646
GTATGCC4106950.057.13728345
GCCGTCT4098050.057.1139649
CGTATGC4110800.057.10757444
ATGCCGT4107250.057.08604447
TGCCGTC4105700.057.06670448
GCTTGAA4105100.057.05649659
TCTCGTA4055250.057.04223641
CCGTCTT4110850.056.9859450
CTCGTAT4078400.056.8832142
TCGTATG4132250.056.8441643
CTTGAAA4129250.056.81728760
TTCTGCT4114800.056.79204655
ATCTCGT4080950.056.59655840
CTGCTTG4128200.056.5806357
TCTGCTT4138450.056.50688656
AATCTCG4071450.056.31742539
TGAAAAA4196550.056.19374562
Produced by FastQC (version 0.11.5)