FastQCFastQC Report
Fri 18 Jan 2019
HGGLTBGX9_n01_3_BZIP3_BIO_5_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGLTBGX9_n01_3_BZIP3_BIO_5_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6889183
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC411794859.77411254716271TruSeq Adapter, Index 3 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG6479059.404671061866116TruSeq Adapter, Index 3 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC398760.578820449391459TruSeq Adapter, Index 3 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTG289810.4206739754191462TruSeq Adapter, Index 3 (97% over 49bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT232590.33761623112639044Illumina Single End PCR Primer 1 (96% over 30bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC211970.30768525092162596TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATTC177670.2578970539757762TruSeq Adapter, Index 3 (98% over 50bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG170680.24775071296552872TruSeq Adapter, Index 3 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATG164050.2381269302905729TruSeq Adapter, Index 3 (97% over 49bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG162480.23584799532832848Illumina Single End PCR Primer 1 (96% over 32bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGT159280.23120303234795767TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCCTATGC147990.21481502233283686TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATGC123380.1790923539119225TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGTCGTATGC123030.17858431108594444TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTAAGC121830.1768424499683054TruSeq Adapter, Index 3 (98% over 50bp)
GATCCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG121350.17614570552124978TruSeq Adapter, Index 3 (97% over 47bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGT119140.17293777796293117Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAGCTCGTATGC116430.1690040749389296TruSeq Adapter, Index 3 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATG114910.16679771752325348TruSeq Adapter, Index 3 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTAGTATGC101530.147375966061578TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGGATCTCGTATGC98050.1423245688204247TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTGC85210.1236866548616868TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG84170.12217704189306627TruSeq Adapter, Index 3 (100% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGAATCTCGTATGC80680.11711112914259934TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTATGCATCTCGTATGC79480.1153692680249603TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATATCGTATGC77120.11194360782693681TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGG72900.10581806289657279TruSeq Adapter, Index 3 (97% over 49bp)
GATCGGAAAAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC70740.10268271288482247TruSeq Adapter, Index 3 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70020.10163759621423904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG3844950.069.0689570
CACGCTA7500.062.99650
AGTCTAC20100.062.52571528
GAATCGG25650.062.2264061
TTCCGAG27850.059.70140525
CTGCTTG5068900.059.58144857
TTCTGCT5067100.059.55660655
TCTGCTT5107300.059.50922456
CTTCTGC5112400.059.49960354
AAAAGCG22300.059.4805370
TCTTCTG5100950.059.470353
TGCTTGA5098950.059.45130558
GTCTTCT5089800.059.4043652
TCTCGTA5201700.058.7258341
CTCGTAT5199700.058.7257142
TCGTATG5201300.058.70800843
CGTATGC5261100.058.5676944
CGTCTTC5198050.058.50255251
TATGCCG5267200.058.43835446
ATGCCGT5245800.058.4378947
Produced by FastQC (version 0.11.5)