FastQCFastQC Report
Fri 18 Jan 2019
HGGLTBGX9_n01_2_NLP7_BIO_3_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGLTBGX9_n01_2_NLP7_BIO_3_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15873774
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC234285714.759294166592015TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG3299522.0785983219869455TruSeq Adapter, Index 2 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC329280.20743649241824913TruSeq Adapter, Index 2 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTTTG223270.1406533821131635TruSeq Adapter, Index 2 (97% over 49bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT203010.12789019170866361Illumina Single End PCR Primer 1 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG2266400.066.699470
TGCTTGA2898450.059.62858658
TCTCGTA2944350.059.36626441
CGTATGC2959550.059.3395444
TCGTATG2932200.059.23880843
CTCGTAT2947450.059.19025842
TATGCCG2969150.059.09097346
GTATGCC2989600.059.0479245
GCCGTCT2933050.058.91478349
ATGCCGT2964500.058.79982447
TCTGCTT2956200.058.7534656
CCGTCTT2967600.058.63991550
CTGCTTG2942200.058.5177657
CTTCTGC2974850.058.50575354
TTCTGCT2952750.058.41246455
ATCTCGT3004700.058.39705340
GCTTGAA2980950.058.3786459
TCTTCTG2974800.058.16882753
TATCTCG3018300.058.0235139
TGCCGTC3005550.057.78038448