Basic Statistics
Measure | Value |
---|---|
Filename | HGGLTBGX9_n01_2_NLP7_BIO_3_R1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 15873774 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC | 2342857 | 14.759294166592015 | TruSeq Adapter, Index 2 (100% over 50bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG | 329952 | 2.0785983219869455 | TruSeq Adapter, Index 2 (100% over 49bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC | 32928 | 0.20743649241824913 | TruSeq Adapter, Index 2 (98% over 50bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTTTG | 22327 | 0.1406533821131635 | TruSeq Adapter, Index 2 (97% over 49bp) |
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT | 20301 | 0.12789019170866361 | Illumina Single End PCR Primer 1 (96% over 30bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAAAGGG | 226640 | 0.0 | 66.6994 | 70 |
TGCTTGA | 289845 | 0.0 | 59.628586 | 58 |
TCTCGTA | 294435 | 0.0 | 59.366264 | 41 |
CGTATGC | 295955 | 0.0 | 59.33954 | 44 |
TCGTATG | 293220 | 0.0 | 59.238808 | 43 |
CTCGTAT | 294745 | 0.0 | 59.190258 | 42 |
TATGCCG | 296915 | 0.0 | 59.090973 | 46 |
GTATGCC | 298960 | 0.0 | 59.04792 | 45 |
GCCGTCT | 293305 | 0.0 | 58.914783 | 49 |
ATGCCGT | 296450 | 0.0 | 58.799824 | 47 |
TCTGCTT | 295620 | 0.0 | 58.75346 | 56 |
CCGTCTT | 296760 | 0.0 | 58.639915 | 50 |
CTGCTTG | 294220 | 0.0 | 58.51776 | 57 |
CTTCTGC | 297485 | 0.0 | 58.505753 | 54 |
TTCTGCT | 295275 | 0.0 | 58.412464 | 55 |
ATCTCGT | 300470 | 0.0 | 58.397053 | 40 |
GCTTGAA | 298095 | 0.0 | 58.37864 | 59 |
TCTTCTG | 297480 | 0.0 | 58.168827 | 53 |
TATCTCG | 301830 | 0.0 | 58.02351 | 39 |
TGCCGTC | 300555 | 0.0 | 57.780384 | 48 |