Basic Statistics
Measure | Value |
---|---|
Filename | HGGLTBGX9_n01_1_NLP7_BIO_1_R1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 14424453 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC | 2862040 | 19.841584287459636 | TruSeq Adapter, Index 1 (100% over 50bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG | 525828 | 3.6453930003446233 | TruSeq Adapter, Index 1 (100% over 49bp) |
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC | 27596 | 0.19131401377924 | TruSeq Adapter, Index 1 (100% over 50bp) |
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT | 15940 | 0.11050679010150334 | Illumina Single End PCR Primer 1 (96% over 30bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC | 15457 | 0.10715830957333357 | TruSeq Adapter, Index 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAAAGGG | 277365 | 0.0 | 67.09523 | 70 |
TATGCCG | 380620 | 0.0 | 56.60934 | 46 |
GTATGCC | 380800 | 0.0 | 56.586514 | 45 |
CGTATGC | 380955 | 0.0 | 56.55929 | 44 |
TCTCGTA | 377075 | 0.0 | 56.544437 | 41 |
GCCGTCT | 380655 | 0.0 | 56.53625 | 49 |
CTCGTAT | 379275 | 0.0 | 56.458897 | 42 |
ATGCCGT | 381750 | 0.0 | 56.428154 | 47 |
TCGTATG | 382440 | 0.0 | 56.39053 | 43 |
ATCTCGT | 376505 | 0.0 | 56.348633 | 40 |
CCGTCTT | 382815 | 0.0 | 56.27074 | 50 |
TGCTTGA | 382455 | 0.0 | 56.257717 | 58 |
TGCCGTC | 383195 | 0.0 | 56.201412 | 48 |
GCTTGAA | 383800 | 0.0 | 56.093777 | 59 |
CGATCTC | 374775 | 0.0 | 56.0628 | 38 |
CTGCTTG | 385330 | 0.0 | 55.795097 | 57 |
TCTGCTT | 386080 | 0.0 | 55.744846 | 56 |
CTTGAAA | 386930 | 0.0 | 55.732273 | 60 |
CTTCTGC | 386510 | 0.0 | 55.727375 | 54 |
GATCTCG | 379535 | 0.0 | 55.721195 | 39 |