FastQCFastQC Report
Fri 18 Jan 2019
HGGLTBGX9_n01_1_NLP7_BIO_1_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGLTBGX9_n01_1_NLP7_BIO_1_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14424453
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC286204019.841584287459636TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG5258283.6453930003446233TruSeq Adapter, Index 1 (100% over 49bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC275960.19131401377924TruSeq Adapter, Index 1 (100% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT159400.11050679010150334Illumina Single End PCR Primer 1 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC154570.10715830957333357TruSeq Adapter, Index 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG2773650.067.0952370
TATGCCG3806200.056.6093446
GTATGCC3808000.056.58651445
CGTATGC3809550.056.5592944
TCTCGTA3770750.056.54443741
GCCGTCT3806550.056.5362549
CTCGTAT3792750.056.45889742
ATGCCGT3817500.056.42815447
TCGTATG3824400.056.3905343
ATCTCGT3765050.056.34863340
CCGTCTT3828150.056.2707450
TGCTTGA3824550.056.25771758
TGCCGTC3831950.056.20141248
GCTTGAA3838000.056.09377759
CGATCTC3747750.056.062838
CTGCTTG3853300.055.79509757
TCTGCTT3860800.055.74484656
CTTGAAA3869300.055.73227360
CTTCTGC3865100.055.72737554
GATCTCG3795350.055.72119539