FastQCFastQC Report
Fri 18 Jan 2019
HGGLTBGX9_n01_16_INPUT_CRF4_5_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGLTBGX9_n01_16_INPUT_CRF4_5_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15483102
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTAT4122962.662877245141187TruSeq Adapter, Index 16 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTA481030.3106806375104937TruSeq Adapter, Index 16 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCGCGTAT174050.11241287437104013TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC509700.059.03555746
GTATGCC514950.058.9498747
TCTCGTA529700.058.8783743
TATGCCG494600.058.67130748
GCGTGAA142650.058.53555361
CGTCCGC631850.058.286635
ATGCCGT499750.058.2737949
CCGTCCG635300.058.24559834
TCCGCAT621850.058.22164537
CCCGTCC638550.058.1260933
GCATCTC561150.058.03087240
CTGCGTG114850.057.98489459
CACCCGT647050.057.87222731
TGCCGTC504850.057.74749850
TCACCCG649300.057.733630
ACCCGTC646000.057.66765632
GCCGTCT483150.057.6455151
GTCACCC654600.057.63980529
CCGTCTT495650.057.01061652
GTCCGCA639400.056.67853536
Produced by FastQC (version 0.11.5)