FastQCFastQC Report
Fri 18 Jan 2019
HGGLTBGX9_n01_15_INPUT_BZIP3_5_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGLTBGX9_n01_15_INPUT_BZIP3_5_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15932519
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1837601.1533643863848522TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTTT332850.20891235089692972TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGAT218600.13720366503250364TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA206070.12933924635520597TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTG40050.057.49366459
CGTATGC181500.056.02255646
ATCTCGT297650.055.98750742
TCTCGTA244800.055.82147643
GTATGCC185550.055.364847
TATGCCG168200.055.31030348
GTCACAT470750.055.1777929
GCGTGAA147750.055.0686461
ATGTCAG465600.055.0542234
ACATGTC469700.054.83033832
ATGCCGT176450.054.5062449
CATGTCA472350.054.448633
TGCCGTC185350.053.55048850
CTGCGTG106600.053.1479259
CTGAACT501650.053.06962619
GCCGTCT173550.052.91557351
CGTCTGA505100.052.62019716
ACGTCTG508500.052.28196315
TCTGCGT111750.052.07644358
CTCCAGT509650.051.9095624
Produced by FastQC (version 0.11.5)