FastQCFastQC Report
Fri 18 Jan 2019
HGGLTBGX9_n01_14_BZIP3_BIO_180_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGLTBGX9_n01_14_BZIP3_BIO_180_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22217338
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT4018941.8089205826548618TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA563620.253684757372823TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG507000.056.23507348
GTATGCC526200.055.8668847
CGTATGC524550.055.6095246
GTTCCGT593800.055.37127335
GCACACG626950.055.3327711
TGCCGTC517300.055.19432450
ATGCCGT517150.055.14303249
ACGTCTG630200.054.99212615
CACACGT635300.054.68813312
ACACGTC635600.054.6849613
CGTCTGA635550.054.52358616
GCCGTCT509600.054.4550751
CACAGTT615300.054.36173231
TCTCGTA554150.054.2705443
TTCCGTA605150.054.14135436
CCGTATC606850.054.0600538
TCACAGT622300.053.84770230
CTCGTAT558450.053.83337444
AGCACAC646650.053.7543410
GTCACAG629500.053.55330729
Produced by FastQC (version 0.11.5)