FastQCFastQC Report
Fri 18 Jan 2019
HGGLTBGX9_n01_13_CRF4_BIO_10_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGLTBGX9_n01_13_CRF4_BIO_10_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33672716
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT7006962.0809013445782036TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA960500.2852457758382187TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG963650.054.2751148
CGTATGC977350.054.2030346
GTATGCC979800.054.03502347
ATGCCGT972700.053.75518849
TGCCGTC976650.053.51587750
GCCGTCT973200.053.4644951
TCTCGTA1012200.052.37766343
CTCGTAT1011750.052.34089744
TCGTATG1024950.051.79240845
CCGTCTT1015950.051.39711452
CACAGTC1067500.050.801931
AGTCAAC1076200.049.80550434
CAGTCAA1085900.049.55653833
ATCTCGT1062700.049.53832642
TCACAGT1100700.049.50065230
ACAGTCA1095850.049.324732
TGCTTGA1036250.048.70743660
ACAATCT1082200.048.602839
GCTTGAA1055150.048.2472461
GTCACAG1135700.048.21811329
Produced by FastQC (version 0.11.5)