FastQCFastQC Report
Fri 18 Jan 2019
HGGLTBGX9_n01_12_BZIP3_BIO_10_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGLTBGX9_n01_12_BZIP3_BIO_10_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99741184
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC23584242.3645438177272893TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG2318660.23246766350798484TruSeq Adapter, Index 12 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2984600.059.574746
CGTATGC2999750.059.2444244
GTATGCC3017450.059.05790745
ATGCCGT3006700.059.02559747
TGCCGTC3014400.058.75091648
GCCGTCT3009900.058.73443249
TCTCGTA3069200.057.20189741
CTCGTAT3079100.057.0273942
TCGTATG3139100.056.5355443
CCGTCTT3143750.056.3900550
ATCTCGT3139650.055.9545840
TGTAATC3152200.055.2007336
AGTCACC3342950.053.5744728
AAAAGGG2689750.053.4194970
TGCTTGA3315250.053.28949458
GTAATCT3279650.053.06123737
CTCCAGT3427350.052.8498624
ACTCCAG3439500.052.7109523
GCTTGAA3376150.052.55338359
CAGTCAC3455150.052.36361727
Produced by FastQC (version 0.11.5)