FastQCFastQC Report
Fri 18 Jan 2019
HGGLTBGX9_n01_11_RAV1_BIO_180_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGLTBGX9_n01_11_RAV1_BIO_180_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16258209
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC322443419.832651923714355TruSeq Adapter, Index 11 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG3499162.152241984341572TruSeq Adapter, Index 11 (100% over 49bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT280190.17233755575414242Illumina Single End PCR Primer 1 (96% over 30bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGT178080.1095323599296823TruSeq Adapter, Index 11 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC163280.10042926622483449TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG2899050.067.0433770
TATGCCG3973000.060.93346446
GTATGCC3973850.060.92489645
CGTATGC3976350.060.8780344
ATGCCGT3979350.060.8277547
GCCGTCT3971700.060.81545649
TGCCGTC3977000.060.78902448
CCGTCTT3985750.060.6570950
TCGTATG3993850.060.6448243
TGCTTGA3980050.060.63329358
TCTCGTA3978500.060.53481341
CTCGTAT3990250.060.49985542
GCTTGAA4003200.060.3781359
ATCTCGT3988800.060.2406940
TTCTGCT4007300.060.13814555
CTTGAAA4029050.060.11532260
TCTGCTT4018800.060.0385256
CTGCTTG4015150.060.02879357
CATCTCG3990450.059.90128739
CTACATC3961450.059.76602636
Produced by FastQC (version 0.11.5)