FastQCFastQC Report
Fri 18 Jan 2019
HGGLTBGX9_n01_10_CRF4_BIO_180_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGLTBGX9_n01_10_CRF4_BIO_180_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18505840
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC3374691.8235810965619503TruSeq Adapter, Index 10 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG489070.2642787357936738TruSeq Adapter, Index 10 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC458750.056.79048544
TATGCCG464150.056.40575446
GTATGCC471550.056.24951645
TCTCGTA463850.055.46293641
ATGCCGT464450.055.10991747
CTCGTAT469450.054.6155242
TCGTATG464650.054.48105643
GCCGTCT463700.054.41909849
TGCCGTC477050.053.51435548
AAAAGGG460550.053.17679670
CCGTCTT487150.052.625350
TATCTCG499250.052.43863739
ATCTCGT509550.051.13023840
TGCTTGA513250.050.29150858
CACTAGC573850.050.0770631
GTCACTA580250.049.80362729
AGTCACT588050.049.368228
ACTAGCT580650.049.29150432
TAGCTTA583300.048.79509434
TCACTAG592700.048.62200530
Produced by FastQC (version 0.11.5)