FastQCFastQC Report
Tue 22 Aug 2017
HGGK5BGX3_n01_5489.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGK5BGX3_n01_5489.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8439
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG7869.313899751155349TruSeq Adapter, Index 2 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG840.9953785993601136TruSeq Adapter, Index 2 (97% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG540.6398862424457874No Hit
AGATCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140.16589643322668562No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA100.11849745230477546TruSeq Adapter, Index 2 (100% over 63bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG200.00716715551.64285769
AAAAGGG200.00716715551.64285767
AAAGGGG200.00716715551.64285768
AAAAAAG1550.051.0875669
GAAAAAA1600.049.4910764
TTGAAAA1600.049.4910762
GCTTGAA1650.047.9913460
CTTGAAA1650.047.9913461
TGAAAAA1650.047.9913463
TGCTTGA1750.045.2489859
TTCTGCT1800.043.9920656
CTGCTTG1800.043.9920658
CTTCTGC1800.043.9920655
TCTGCTT1850.042.8030957
CGTCTTC1850.042.8030952
GTCTTCT1900.041.6766953
CACCGAT2100.040.98639332
CAGTCAC2200.040.93195328
CCGTCTT1950.040.6080651
GTCACCG2050.040.54797730
Produced by FastQC (version 0.11.2)