HGGK5BGX3_n01_5488.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HGGK5BGX3_n01_5488.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40978323
Sequences flagged as poor quality	0
Sequence length	75
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG	1682310	4.1053656588142955	TruSeq Adapter, Index 21 (98% over 63bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG	88242	0.21533824114764286	TruSeq Adapter, Index 21 (98% over 59bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCCGTCTTCTGCTT	69609	0.16986785916056155	TruSeq Adapter, Index 21 (98% over 62bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG	50126	0.12232320976141459	TruSeq Adapter, Index 21 (98% over 61bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
AAAAAAG	281760	46.70581	69
GAAAAAA	310060	43.39718	64
CGTGTGC	31830	42.881252	1
GCTTGAA	320045	41.63845	60
CTTGAAA	330325	40.42195	61
TGCTTGA	333650	39.876534	59
TGAAAAA	345605	38.815052	63
TTGAAAA	348140	38.470177	62
CTGCTTG	348625	38.121105	58
AGTCGGA	11790	35.35988	1
TCTGCTT	377005	35.320118	57
CTTCTGC	383190	34.812958	55
TTCTGCT	385040	34.60358	56
CGTCTTC	388025	34.364685	52
GTCTTCT	390390	34.16698	53
GCCGTCT	391495	34.015778	50
CCGTCTT	392000	33.962654	51
TCTTCTG	396810	33.63051	54
TGCCGTC	409025	32.60379	49
GTGTGCT	41870	32.548294	2