FastQCFastQC Report
Tue 22 Aug 2017
HGGK5BGX3_n01_5487.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGK5BGX3_n01_5487.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24417969
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG8712713.568155074650148TruSeq Adapter, Index 8 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2231900.9140399842427517TruSeq Adapter, Index 8 (100% over 63bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG352220.14424623112593843TruSeq Adapter, Index 8 (100% over 59bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTT268950.11014429578479684TruSeq Adapter, Index 8 (100% over 62bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG261980.10728984052686774TruSeq Adapter, Index 8 (100% over 60bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG255630.10468929664051911No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC296400.045.1887631
AAAAAAG1707750.039.81453369
ACGTGTG107050.038.493081
GTGTGCT358750.037.343042
AAAAAGG642600.037.317169
GAAAAAA1861800.036.9997964
TGAAAAA1944000.035.31498763
CTTGAAA1926850.035.26248661
GCTTGAA1936750.034.936160
TTGAAAA1974150.034.52938562
TGCTTGA1980900.033.90875259
CTGCTTG2069850.032.3581558
TCTGCTT2246150.029.9277257
CTTCTGC2247600.029.91295455
TTCTGCT2270350.029.5007156
CGTCTTC2288050.029.4339552
GTCTTCT2287200.029.33043553
AGTCGGA67450.029.1647241
CCGTCTT2317150.029.10926651
TCTTCTG2318900.028.97696754
Produced by FastQC (version 0.11.2)