FastQCFastQC Report
Tue 22 Aug 2017
HGGK5BGX3_n01_5486.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGK5BGX3_n01_5486.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35518147
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG21828986.1458667874762725TruSeq Adapter, Index 20 (98% over 63bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCCGTCTTCTGCTTGA1668600.46978802131766617TruSeq Adapter, Index 20 (98% over 63bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCCGTCTTCTGCTT853060.2401758177305815TruSeq Adapter, Index 20 (98% over 62bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG733220.20643531882448712No Hit
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCCGTCTTCTGCTTG659470.1856712851602309TruSeq Adapter, Index 20 (98% over 63bp)
AAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG544080.1531836669294713TruSeq Adapter, Index 20 (98% over 57bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG394250.11099959691027801TruSeq Adapter, Index 20 (98% over 63bp)
AGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGG360520.10150304293745954TruSeq Adapter, Index 20 (98% over 56bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG3289600.051.54308769
TCGGGGG49250.047.2154664
GAAAAAA3715600.046.80425664
CTTGAAA3804350.045.27580661
GCTTGAA3827500.044.88755460
TGAAAAA3881600.044.64544763
TTGAAAA3904050.044.21034262
GATCGGG68900.039.158452
ACGTGTG268900.038.155541
CGGGGGG62400.037.652395
ATCGGGG62450.036.461833
CGTGTGC524700.035.0434341
TGCTTGA5258000.032.59949559
GTGTGCT611100.030.0187322
CTGCTTG5976200.028.65239558
TGTGCTC991450.025.8207911
TCTGCTT6696350.025.5968857
TTCTGCT6796300.025.202256
CTTCTGC6819050.025.18446455
GTGCTCT1034550.024.8252052
Produced by FastQC (version 0.11.2)