Basic Statistics
Measure | Value |
---|---|
Filename | HGGK5BGX3_n01_5485.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 24118723 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG | 2218069 | 9.196461189093636 | RNA PCR Primer, Index 24 (100% over 43bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 80578 | 0.3340889979954577 | No Hit |
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA | 31415 | 0.1302515062675582 | RNA PCR Primer, Index 24 (100% over 43bp) |
AGATCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 25021 | 0.10374098164318236 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAAAAAG | 291985 | 0.0 | 57.667366 | 69 |
GAAAAAA | 315060 | 0.0 | 54.628353 | 64 |
GCTTGAA | 311490 | 0.0 | 54.62716 | 60 |
TCGGGGG | 4675 | 0.0 | 54.315384 | 4 |
CTTGAAA | 317300 | 0.0 | 53.75193 | 61 |
TGCTTGA | 322495 | 0.0 | 52.65318 | 59 |
TGAAAAA | 328730 | 0.0 | 52.17649 | 63 |
TTGAAAA | 330310 | 0.0 | 51.766068 | 62 |
CTGCTTG | 353445 | 0.0 | 47.967457 | 58 |
GATCGGG | 5610 | 0.0 | 46.862164 | 2 |
ATCGGGG | 5385 | 0.0 | 46.8332 | 3 |
CGGGGGG | 5685 | 0.0 | 45.51531 | 5 |
TCTGCTT | 378320 | 0.0 | 44.903816 | 57 |
TTCTGCT | 389170 | 0.0 | 43.606693 | 56 |
CTTCTGC | 393085 | 0.0 | 43.25691 | 55 |
TCTTCTG | 404355 | 0.0 | 42.038906 | 54 |
GTCTTCT | 405070 | 0.0 | 41.969135 | 53 |
CGTCTTC | 406535 | 0.0 | 41.85515 | 52 |
CCGTCTT | 407115 | 0.0 | 41.771206 | 51 |
GCCGTCT | 411500 | 0.0 | 41.320816 | 50 |