FastQCFastQC Report
Tue 22 Aug 2017
HGGK5BGX3_n01_5485.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGK5BGX3_n01_5485.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24118723
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG22180699.196461189093636RNA PCR Primer, Index 24 (100% over 43bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG805780.3340889979954577No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA314150.1302515062675582RNA PCR Primer, Index 24 (100% over 43bp)
AGATCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG250210.10374098164318236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG2919850.057.66736669
GAAAAAA3150600.054.62835364
GCTTGAA3114900.054.6271660
TCGGGGG46750.054.3153844
CTTGAAA3173000.053.7519361
TGCTTGA3224950.052.6531859
TGAAAAA3287300.052.1764963
TTGAAAA3303100.051.76606862
CTGCTTG3534450.047.96745758
GATCGGG56100.046.8621642
ATCGGGG53850.046.83323
CGGGGGG56850.045.515315
TCTGCTT3783200.044.90381657
TTCTGCT3891700.043.60669356
CTTCTGC3930850.043.2569155
TCTTCTG4043550.042.03890654
GTCTTCT4050700.041.96913553
CGTCTTC4065350.041.8551552
CCGTCTT4071150.041.77120651
GCCGTCT4115000.041.32081650