HGGK5BGX3_n01_5484.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HGGK5BGX3_n01_5484.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25799311
Sequences flagged as poor quality	0
Sequence length	75
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG	3047928	11.813989916242337	RNA PCR Primer, Index 23 (100% over 43bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	126244	0.4893308972476048	No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	44293	0.17168287943813693	RNA PCR Primer, Index 23 (100% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG	39925	0.154752194738844	RNA PCR Primer, Index 23 (97% over 43bp)
AGATCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	38263	0.14831016223650315	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG	36076	0.1398331916693434	RNA PCR Primer, Index 23 (100% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG	27266	0.1056849929054307	RNA PCR Primer, Index 23 (97% over 43bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
AAAAAAG	394190	59.396603	69
TCGGGGG	6850	56.15801	4
GAAAAAA	429085	55.7472	64
GCTTGAA	445175	53.279804	60
CTTGAAA	447440	53.113415	61
TGAAAAA	460690	51.799477	63
TTGAAAA	460500	51.674213	62
ATCGGGG	7330	51.444252	3
TGCTTGA	459955	51.441074	59
GATCGGG	7985	49.51609	2
CTGCTTG	485405	48.691402	58
CGGGGGG	8195	47.488216	5
TCTGCTT	511770	46.233692	57
TTCTGCT	522950	45.218536	56
CTTCTGC	528390	44.841133	55
GTCTTCT	540360	43.874252	53
TCTTCTG	540340	43.84833	54
CGTCTTC	542330	43.758686	52
CCGTCTT	544105	43.583767	51
GCCGTCT	548395	43.2515	50