FastQCFastQC Report
Tue 22 Aug 2017
HGGK5BGX3_n01_5482.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGK5BGX3_n01_5482.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25158410
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG6353732.5254894884056664TruSeq Adapter, Index 16 (98% over 63bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTT462330.18376757513690253TruSeq Adapter, Index 16 (98% over 62bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG1119650.045.5120269
CGTGTGC306500.044.2961921
GAAAAAA1229600.042.6461664
CTTGAAA1278850.040.59123661
GCTTGAA1275050.040.57700360
TGAAAAA1378700.037.91664563
TTGAAAA1391450.037.4726762
TGCTTGA1412450.036.53702559
GTGTGCT374850.036.2371372
CTGCTTG1465300.035.1344658
ACGTGTG115150.032.728131
TCTGCTT1668050.030.86608757
TTCTGCT1701850.030.21663556
CTTCTGC1714850.030.08437255
CGTCTTC1738900.029.65878152
GTCTTCT1742350.029.56423853
TCTTCTG1795450.028.69180354
CCGTCTT1806850.028.49578751
GCCGTCT1813500.028.43325850
CGGGGGG20150.027.0529795
Produced by FastQC (version 0.11.2)