FastQCFastQC Report
Tue 22 Aug 2017
HGGK5BGX3_n01_5481.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGK5BGX3_n01_5481.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17132526
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG14134778.250254515883999TruSeq Adapter, Index 1 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG557660.3254978279326703No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA491990.2871672279966188TruSeq Adapter, Index 1 (100% over 40bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA225740.13176107247699492TruSeq Adapter, Index 1 (100% over 63bp)
AGATCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG176060.10276359714848819No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG1928800.056.45479269
GCTTGAA1979600.055.4829560
TCGGGGG31250.054.8703654
CTTGAAA2036300.054.03969661
TGCTTGA2031200.053.9482659
GAAAAAA2094200.052.911964
TGAAAAA2138950.051.66695463
TTGAAAA2147500.051.33448462
CTGCTTG2158350.050.7445658
TCTGCTT2294400.047.81240557
ATCGGGG36000.046.959123
TTCTGCT2358650.046.52219856
CTTCTGC2377350.046.22618555
GATCGGG38850.045.4690062
CCGTCTT2426350.045.29268351
CGTCTTC2443650.045.00847252
GCCGTCT2443650.044.94766650
GTCTTCT2449900.044.8894353
TCTTCTG2452750.044.82025554
CGGGGGG39900.044.0995
Produced by FastQC (version 0.11.2)