FastQCFastQC Report
Tue 22 Aug 2017
HGGK5BGX3_n01_5480.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGK5BGX3_n01_5480.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21567427
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG13739796.370620844109036TruSeq Adapter, Index 14 (98% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG589050.2731202011255214No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA224410.1040504275266586TruSeq Adapter, Index 14 (98% over 63bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG1919800.055.0130369
GCTTGAA2039350.052.39586660
TCGGGGG27200.052.1315424
GAAAAAA2102950.051.38822664
TGCTTGA2092750.050.96187259
CTTGAAA2123400.050.4241361
TGAAAAA2202800.048.88670763
TTGAAAA2212850.048.5259362
CTGCTTG2253800.047.24527758
CGGGGGG33800.043.6871535
TCTGCTT2457450.043.3805857
TTCTGCT2559850.041.6385256
CTTCTGC2566750.041.60473355
CCGTCTT2602400.041.02835551
GCCGTCT2622850.040.73787350
CGTCTTC2629100.040.64721752
GTCTTCT2652900.040.26063553
TCTTCTG2661900.040.1140554
CGTGTGC223350.040.1136741
ATCGGGG34400.039.9158363
Produced by FastQC (version 0.11.2)