HGGK5BGX3_n01_129.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HGGK5BGX3_n01_129.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16288423
Sequences flagged as poor quality	0
Sequence length	75
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG	1064225	6.5336282094343945	TruSeq Adapter, Index 22 (98% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	81536	0.5005763909741292	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG	50777	0.3117367470135077	TruSeq Adapter, Index 22 (98% over 63bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	30938	0.18993858398692126	TruSeq Adapter, Index 22 (98% over 60bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCCGTCTTCTGCTT	22351	0.13722015937331689	TruSeq Adapter, Index 22 (98% over 62bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG	18686	0.11471951581807521	TruSeq Adapter, Index 22 (98% over 61bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
AAAAAAG	157580	52.610153	69
GAAAAAA	172070	49.3225	64
GCTTGAA	191340	43.907463	60
CTTGAAA	193030	43.61571	61
TGAAAAA	198345	42.67937	63
TTGAAAA	198885	42.435394	62
TGCTTGA	201415	41.599823	59
TCGGGGG	2430	40.60528	4
CTGCTTG	208210	40.199245	58
TCTGCTT	222525	37.655212	57
AAGGGGG	6775	37.426292	69
TTCTGCT	226855	36.943974	56
CTTCTGC	227345	36.90369	55
GTCTTCT	232115	36.13078	53
CGTCTTC	232720	36.07984	52
CGGGGGG	2845	36.015903	5
TCTTCTG	233895	35.840847	54
AGTCGGA	4585	35.753487	1
CGTGTGC	13105	35.472736	1
CCGTCTT	238150	35.232784	51