FastQCFastQC Report
Tue 22 Aug 2017
HGGK5BGX3_n01_128.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGK5BGX3_n01_128.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12353073
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG3674462.974531114646534TruSeq Adapter, Index 15 (98% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG567430.45934319339001717No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG132940.10761694681153426TruSeq Adapter, Index 15 (98% over 63bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG641300.045.4029369
GAAAAAA703900.042.35951264
GCTTGAA715000.041.20992760
TGCTTGA740600.039.72970259
CTGCTTG750200.039.1799158
CTTGAAA758050.038.9879261
TGAAAAA814450.036.53371463
TTGAAAA817800.036.3334462
TCTGCTT820200.035.8951357
CTTCTGC839500.035.1771855
TTCTGCT845350.034.87632856
CGTCTTC846300.034.8215852
GCCGTCT848650.034.6446950
CCGTCTT850700.034.57672551
GTCTTCT853700.034.49967253
TGCCGTC882600.033.34763749
TCTTCTG884800.033.29860354
CGTGTGC69450.032.9460951
ATGCCGT898400.032.76115848
TATGCCG929550.031.6818647
Produced by FastQC (version 0.11.2)