FastQCFastQC Report
Tue 22 Aug 2017
HGGK5BGX3_n01_127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGK5BGX3_n01_127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19688937
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG6232213.165335944749074TruSeq Adapter, Index 13 (98% over 63bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTTGA2057101.0447999300317736TruSeq Adapter, Index 13 (98% over 63bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTT1063120.5399580485223758TruSeq Adapter, Index 13 (98% over 62bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTTG801860.40726424184302074TruSeq Adapter, Index 13 (98% over 63bp)
AAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG516510.26233513774766004TruSeq Adapter, Index 13 (98% over 57bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG318550.16179136537437241TruSeq Adapter, Index 13 (98% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG272990.13865146706498174TruSeq Adapter, Index 13 (98% over 62bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG204170.10369782787156055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG1121050.041.80267769
ACGTGTG212800.040.803781
GAAAAAA1331500.035.8330364
TGCTTGA3294950.035.2498369
AGGGGGG123300.032.9595667
CGTGTGC382550.032.792731
TGAAAAA1492450.031.90924563
CTTGAAA1492450.031.7407461
TTGAAAA1532150.030.99694862
TCGGGGG14150.029.7456574
CTGCTTG3942300.029.4474468
TGTGCTC780600.029.4093251
GCTTGAA1631100.028.99611760
GTGCTCT805750.028.5597572
AAGGGGG147150.028.0394966
GACGTGT88500.027.921011
ATCTCGG99750.027.8858261
GTGTGCT447250.027.8320472
CTCTTCC2104900.026.3528061
TCTCGGG33800.025.9266282
Produced by FastQC (version 0.11.2)